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To improve the chicken embryo development rate of in vitro culture and surrogate egg-shell culture, and percentage of embryo collection, the effect of donor hens and quality of embryos were studied. In this study, the embryo collection rates were 86.8% for commercial brown layers and 77.1% for Korea Native Chicken, respectively. The difference was significant between the two breeds(p$lt;0.05). For commercial layers, the embryo development rate by in vitro culture and surrogate egg-shell culture for different regions of magnum of donor hens tended higher at 10 ㎝ to 14 ㎝ of magnum than other regions. However, for Korea Native Chicken the highest embryo development rate was found at 5 ㎝ to 9 ㎝. For efficient collection of single cell stage embryo, high yielding commercial layers are recommended to be selected as experimental chick. The donor layers must have a regular ovulation time and have laying at the day of egg collection. To increase the embryos, we suggest the process of 1st and 2nd culture of exchanging culture media and vessel is not necessary.
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